Chinese Birds
Chinese Birds 2012, 3(2) 103-107 DOI:   10.5122/cbirds.2012.0010  ISSN: 1674-7674 CN: 11-5870/Q

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Keywords
Crossoptilon auritum
microsatellites
cross-species amplification
Z-chromosomal linkage
Authors
Langyu GU
Yang LIU
Ning WANG
Zhengwang ZHANG
PubMed
Article by Langyu GU
Article by Yang LIU
Article by Ning WANG
Article by Zhengwang ZHANG

A panel of polymorphic microsatellites in the Blue Eared Pheasant (Crossoptilon auritum) developed by cross-species amplification

Langyu GU 1, Yang LIU 2,*, Ning WANG 1, Zhengwang ZHANG 1

1 MOE Key Laboratory for Biodiversity Sciences and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China

2 Computational and Molecular Population Genetics, Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012, Bern, Switzerland

Abstract

Polymorphic microsatellites are among the versatile genetic markers in molecular ecology studies. In contrast to de novo isolation of microsatellites from target species, cross-species amplification is a cost-effective approach for a fast development of microsatellite markers from closely related taxa. In our study, we cross-amplified a panel of polymorphic microsatellite markers for the Blue Eared Pheasant (Crossoptilon auritum), a species endemic to China of considerable conservation concern. We obtained 11 polymorphic microsatellite markers selected from 112 candidate loci, originally isolated from other Galliforme species. This panel of makers has shown moderate to high levels of polymorphism and include a Z-chromosomal linkage locus. We carried out preliminary analyses of parentage among captive individuals with a known pedigree using this new panel of microsatellites. Our results suggest that the high utility of these markers may be powerful tools for studies in conservation genetics of eared-pheasants and other endangered Galliforme species.

Keywords Crossoptilon auritum   microsatellites   cross-species amplification   Z-chromosomal linkage  
Received 2012-03-04 Revised 2012-05-10 Online:  
DOI: 10.5122/cbirds.2012.0010
Corresponding Authors: Zhengwang ZHANG
Email: zzw@bnu.edu.cn
About author:

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